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Secondly, although the mechanism proposed in this study was validated in BMSCs, the in vivo environment is complex and affected by multiple factors, involving multiple pathways and metabolism, making it difficult to conclude that the GLP-1-activated Wnt pathway plays a decisive role in osteogenesis

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Generation of lag-1 CRIPSR alleles and transgenes expressing lag-1 and BirA lag-1(oz530[lag-1 :: 3xHA]) ( Fig 2A ) was generated through in vitro assembled RNA protein complex (RNP) CRISPR [65,66]

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