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For quantification of TSNAs, LCMS/MS was run with mobile phase A (water: 0.1% formic acid (2:98 v/v) with pH = 3) and mobile phase B (100% methanol: 0.1% formic acid with pH = 3) at a flow rate of 0.5 ml/min (isocratic flow) coupled to a triple quadrupole Mass spectrometer with a capillary voltage of 4000 V, Nozzle voltage of 500 V, nebulizer gas nitrogen with a pressure of 45 psi, the gas temperature of 300 C and sheath gas temperature of 350 C and gas flow and sheath gas flow of 8L/min and 11L/min, respectively

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